diff --git a/Userland/Libraries/LibWeb/DOM/Document.cpp b/Userland/Libraries/LibWeb/DOM/Document.cpp
index 9e9281f547..9549165a75 100644
--- a/Userland/Libraries/LibWeb/DOM/Document.cpp
+++ b/Userland/Libraries/LibWeb/DOM/Document.cpp
@@ -1,5 +1,5 @@
 /*
- * Copyright (c) 2018-2023, Andreas Kling <kling@serenityos.org>
+ * Copyright (c) 2018-2024, Andreas Kling <kling@serenityos.org>
  * Copyright (c) 2021-2023, Linus Groh <linusg@serenityos.org>
  * Copyright (c) 2021-2023, Luke Wilde <lukew@serenityos.org>
  * Copyright (c) 2021-2023, Sam Atkins <atkinssj@serenityos.org>
@@ -52,6 +52,7 @@
 #include <LibWeb/DOM/Text.h>
 #include <LibWeb/DOM/TreeWalker.h>
 #include <LibWeb/Dump.h>
+#include <LibWeb/FileAPI/BlobURLStore.h>
 #include <LibWeb/HTML/AttributeNames.h>
 #include <LibWeb/HTML/BrowsingContext.h>
 #include <LibWeb/HTML/CustomElements/CustomElementDefinition.h>
@@ -2988,6 +2989,8 @@ void Document::run_unloading_cleanup_steps()
         // 2. Clear window's map of active timers.
         window->clear_map_of_active_timers();
     }
+
+    FileAPI::run_unloading_cleanup_steps(*this);
 }
 
 // https://html.spec.whatwg.org/multipage/document-lifecycle.html#destroy-a-document
diff --git a/Userland/Libraries/LibWeb/FileAPI/BlobURLStore.cpp b/Userland/Libraries/LibWeb/FileAPI/BlobURLStore.cpp
index 08e2adb9e7..fdc119592f 100644
--- a/Userland/Libraries/LibWeb/FileAPI/BlobURLStore.cpp
+++ b/Userland/Libraries/LibWeb/FileAPI/BlobURLStore.cpp
@@ -1,5 +1,6 @@
 /*
  * Copyright (c) 2023, Tim Flynn <trflynn89@serenityos.org>
+ * Copyright (c) 2024, Andreas Kling <kling@serenityos.org>
  *
  * SPDX-License-Identifier: BSD-2-Clause
  */
@@ -7,6 +8,7 @@
 #include <AK/StringBuilder.h>
 #include <LibURL/URL.h>
 #include <LibWeb/Crypto/Crypto.h>
+#include <LibWeb/DOM/Document.h>
 #include <LibWeb/FileAPI/Blob.h>
 #include <LibWeb/FileAPI/BlobURLStore.h>
 #include <LibWeb/HTML/Origin.h>
@@ -89,4 +91,19 @@ ErrorOr<void> remove_entry_from_blob_url_store(StringView url)
     return {};
 }
 
+// https://w3c.github.io/FileAPI/#lifeTime
+void run_unloading_cleanup_steps(JS::NonnullGCPtr<DOM::Document> document)
+{
+    // 1.  Let environment be the Document's relevant settings object.
+    auto& environment = document->relevant_settings_object();
+
+    // 2.  Let store be the user agent’s blob URL store;
+    auto& store = FileAPI::blob_url_store();
+
+    // 3. Remove from store any entries for which the value's environment is equal to environment.
+    store.remove_all_matching([&](auto&, auto& value) {
+        return value.environment == &environment;
+    });
+}
+
 }
diff --git a/Userland/Libraries/LibWeb/FileAPI/BlobURLStore.h b/Userland/Libraries/LibWeb/FileAPI/BlobURLStore.h
index 6e83cd076d..13f39c824c 100644
--- a/Userland/Libraries/LibWeb/FileAPI/BlobURLStore.h
+++ b/Userland/Libraries/LibWeb/FileAPI/BlobURLStore.h
@@ -28,4 +28,6 @@ ErrorOr<String> generate_new_blob_url();
 ErrorOr<String> add_entry_to_blob_url_store(JS::NonnullGCPtr<Blob> object);
 ErrorOr<void> remove_entry_from_blob_url_store(StringView url);
 
+void run_unloading_cleanup_steps(JS::NonnullGCPtr<DOM::Document>);
+
 }