diff --git a/.github/workflows/run-tests.yml b/.github/workflows/run-tests.yml
index 82c0f7136..31f4500a9 100644
--- a/.github/workflows/run-tests.yml
+++ b/.github/workflows/run-tests.yml
@@ -28,7 +28,7 @@ jobs:
         pip install -r requirements.txt
     - name: Check coding style
       run: |
-        pip install flake8 "isort<5"
+        pip install flake8 "isort>=5"
         flake8 picard test --count --show-source --statistics
         isort --check-only --diff --recursive picard test
     - name: Test with pytest
diff --git a/.isort.cfg b/.isort.cfg
deleted file mode 100644
index 200f3af5d..000000000
--- a/.isort.cfg
+++ /dev/null
@@ -1,28 +0,0 @@
-# Configuration for isort tool
-# https://github.com/timothycrosley/isort
-#
-# Possible settings are listed at https://github.com/timothycrosley/isort/wiki/isort-Settings
-#
-# It can easily be installed using `pip install isort`
-#
-[settings]
-combine_as_imports=True
-default_section=LOCALFOLDER
-force_grid_wrap=True
-force_sort_within_sections=True
-include_trailing_comma=True
-indent='    '
-known_picard=picard
-known_picard_ui=picard.ui
-known_qt=PyQt5
-known_resources=picard.resources
-known_test=test.*
-known_third_party=mutagen
-multi_line_output=3
-order_by_type=True
-sections=FUTURE,STDLIB,THIRDPARTY,QT,TEST,PICARD,LOCALFOLDER,RESOURCES,PICARD_UI
-skip_glob=**/ui_*.py
-skip=./tagger.py,picard/resources.py,scripts/picard.in,picard/const/__init__.py
-not_skip=__init__.py
-use_parentheses=1
-lines_after_imports=2
diff --git a/picard/__init__.py b/picard/__init__.py
index 7cd23fd67..cd3da4593 100644
--- a/picard/__init__.py
+++ b/picard/__init__.py
@@ -102,8 +102,15 @@ def crash_handler():
 
     # Display the crash information to the user as a dialog. This requires
     # importing Qt5 and has some potential to fail if things are broken.
-    from PyQt5.QtCore import QCoreApplication, Qt, QUrl
-    from PyQt5.QtWidgets import QApplication, QMessageBox
+    from PyQt5.QtCore import (
+        QCoreApplication,
+        Qt,
+        QUrl,
+    )
+    from PyQt5.QtWidgets import (
+        QApplication,
+        QMessageBox,
+    )
     app = QCoreApplication.instance()
     if not app:
         app = QApplication(sys.argv)
diff --git a/picard/const/__init__.py b/picard/const/__init__.py
index 0229dd84f..cdd68dab3 100644
--- a/picard/const/__init__.py
+++ b/picard/const/__init__.py
@@ -36,8 +36,9 @@ import builtins
 from collections import OrderedDict
 
 from picard import PICARD_VERSION
-from picard.const.attributes import MB_ATTRIBUTES
 from picard.const import appdirs
+from picard.const.attributes import MB_ATTRIBUTES
+
 
 # Install gettext "noop" function in case const.py gets imported directly.
 builtins.__dict__['N_'] = lambda a: a
@@ -112,17 +113,23 @@ for k, v in MB_ATTRIBUTES.items():
     elif k.startswith('DB:release_status/name:'):
         RELEASE_STATUS[v] = v
 
-# List of available charsets
-from picard.const.scripts import SCRIPTS  # noqa: F401,E402 # pylint: disable=unused-import
-
 # Release countries
-from picard.const.countries import RELEASE_COUNTRIES  # noqa: F401,E402 # pylint: disable=unused-import
-
+from picard.const.countries import (  # noqa: F401,E402 # pylint: disable=unused-import
+    RELEASE_COUNTRIES,
+)
 # List of available user interface languages
-from picard.const.languages import UI_LANGUAGES  # noqa: F401,E402 # pylint: disable=unused-import
-
+from picard.const.languages import (  # noqa: F401,E402 # pylint: disable=unused-import
+    UI_LANGUAGES,
+)
 # List of alias locales
-from picard.const.locales import ALIAS_LOCALES  # noqa: F401,E402 # pylint: disable=unused-import
+from picard.const.locales import (  # noqa: F401,E402 # pylint: disable=unused-import
+    ALIAS_LOCALES,
+)
+# List of available charsets
+from picard.const.scripts import (  # noqa: F401,E402 # pylint: disable=unused-import
+    SCRIPTS,
+)
+
 
 # List of official musicbrainz servers - must support SSL for mblogin requests (such as collections).
 MUSICBRAINZ_SERVERS = [
diff --git a/picard/formats/mutagenext/tak.py b/picard/formats/mutagenext/tak.py
index 17afc50ee..8aa29db98 100644
--- a/picard/formats/mutagenext/tak.py
+++ b/picard/formats/mutagenext/tak.py
@@ -34,11 +34,11 @@ __all__ = ["TAK", "Open", "delete"]
 
 try:
     from mutagen.tak import (
-        Open,
         TAK,
+        Open,
         TAKHeaderError,
         TAKInfo,
-        delete
+        delete,
     )
 
     native_tak = True
diff --git a/picard/formats/wav.py b/picard/formats/wav.py
index 515fa79e7..77c91ef41 100644
--- a/picard/formats/wav.py
+++ b/picard/formats/wav.py
@@ -36,10 +36,10 @@ from picard.metadata import Metadata
 
 
 try:
-    import mutagen.wave
     from mutagen._iff import assert_valid_chunk_id
     from mutagen._riff import RiffFile
     from mutagen._util import loadfile
+    import mutagen.wave
 
     # See https://exiftool.org/TagNames/RIFF.html
     TRANSLATE_RIFF_INFO = {
diff --git a/picard/util/filenaming.py b/picard/util/filenaming.py
index 2c2472725..3d27ad190 100644
--- a/picard/util/filenaming.py
+++ b/picard/util/filenaming.py
@@ -55,8 +55,8 @@ from picard.util import (
 win32api = None
 if IS_WIN:
     try:
-        import win32api  # isort:skip
         import pywintypes
+        import win32api
     except ImportError as e:
         log.warning('pywin32 not available: %s', e)
 
diff --git a/picard/util/imagelist.py b/picard/util/imagelist.py
index c09366e88..4ece144f0 100644
--- a/picard/util/imagelist.py
+++ b/picard/util/imagelist.py
@@ -165,6 +165,7 @@ def _update_state(obj, state):
 # TODO: use functools.singledispatch when py3 is supported
 def _get_state(obj):
     from picard.album import Album
+
     from picard.ui.item import FileListItem
 
     state = ImageListState()
diff --git a/picard/util/pipe.py b/picard/util/pipe.py
index d0899c5cc..aa35168be 100644
--- a/picard/util/pipe.py
+++ b/picard/util/pipe.py
@@ -48,9 +48,9 @@ from picard.util import sanitize_filename
 
 
 if IS_WIN:
-    import win32pipe  # type: ignore
-    import win32file  # type: ignore
     from pywintypes import error as WinApiError  # type: ignore
+    import win32file  # type: ignore
+    import win32pipe  # type: ignore
 
 
 class PipeError(Exception):
diff --git a/pyproject.toml b/pyproject.toml
index c546bd399..a87c87308 100644
--- a/pyproject.toml
+++ b/pyproject.toml
@@ -1,3 +1,27 @@
 [build-system]
 requires = ["setuptools>=62.4.0"]
 build-backend = "setuptools.build_meta"
+
+[tool.isort]
+sections = [
+    "FUTURE", "STDLIB", "THIRDPARTY", "QT", "TEST", "PICARD", "LOCALFOLDER",
+    "RESOURCES", "PICARD_UI"
+]
+default_section = "LOCALFOLDER"
+known_picard = ["picard"]
+known_picard_ui = ["picard.ui"]
+known_qt = ["PyQt5"]
+known_resources = ["picard.resources"]
+known_test = ["test.*"]
+known_third_party = ["dateutil", "fasteners", "mutagen", "yaml"]
+skip_glob = ["**/ui_*.py", "picard/resources.py"]
+combine_as_imports = true
+float_to_top = false
+force_grid_wrap = 2
+force_sort_within_sections = true
+include_trailing_comma = true
+indent = "    "
+lines_after_imports = 2
+multi_line_output = 3
+order_by_type = true
+use_parentheses = true
diff --git a/requirements-dev.txt b/requirements-dev.txt
index b0697c7d3..3ba0fb999 100644
--- a/requirements-dev.txt
+++ b/requirements-dev.txt
@@ -1,7 +1,7 @@
 autopep8
 Babel>=2.6.0
 flake8
-isort>=4.3.10,<5
+isort>=5.0
 pycodestyle
 pylint>=2.6.0
 transifex-client